Selective isolation methods and distribution of soil actinomycetes.
نویسندگان
چکیده
منابع مشابه
Selective isolation of aerobic Actinomycetes.
The composition of an arginine-glycerol-salt medium (AGS), suitable for the selective isolation of aerobic actinomycetes, was given. When soil samples were treated with calcium carbonate and plated on the AGS medium, higher total and relative plate counts of actinomycetes were obtained than when other media and methods were used.
متن کاملNovel method for selective isolation of actinomycetes.
A new technique for the selective isolation of actinomycetes from natural mixed microbial populations is described. A nutrient agar medium was overlaid with a 0.22- to 0.45-microns-pore cellulose ester membrane filter, and the surface of the filter was inoculated. During incubation, the branched mycelia of the actinomycetes penetrated the filter pores to the underlying agar medium, whereas grow...
متن کاملUse of Antibiotics for Selective Isolation and Enumeration of Actinomycetes in Soil.
Several antibiotics were tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these tests four antibiotics, nystatin (50 pg./ml.), actidione (50 pg./ml.), polymyxin B sulphate (5.0 pg./ml.) and sodium penicillin (1.0 ,ug./ml.), were selected for incorporation into a starch +casein medium to achieve selective growth of actinomycetes on soil dilution ...
متن کاملSelective isolation and distribution of sporichthya strains in soil
A simplified enrichment method in which centrifugation is used for selective isolation of Sporichthya strains from soil is described. Gellan gum plus 2 mM CaCl2 stimulated growth of Sporichthya polymorpha KCC A0089 so that this organism was readily recognized on an isolation plate. High yields of motile spores were obtained by using a flooding solution containing 0.1% skim milk in 10 mM MOPS (m...
متن کاملIsolation, antifungal activity and characterization of soil actinomycetes
Actinomycetes were isolated from eight samples of two different habitats, i.e., garden soil and cultivated field soil. Isolation of actinomycetes was carried out on soil extract agar medium using serial dilution method and forty seven isolates were obtained. All these actinomycetes were then assessed for their antifungal activity against two fungal pathogens i.e., Candida albicans and Aspergill...
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ژورنال
عنوان ژورنال: Actinomycetologica
سال: 1990
ISSN: 1881-6371,0914-5818
DOI: 10.3209/saj.4_103